Chromosome Conformation Capture assays: 3C and HiC

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1. Introduction, overview of the procedure and materials

   A. Introduction
   B. Overview of the procedure
   C. Materials

2. Preparation of 3C DNA

   A. Cell lysis, nuclei preparation and restriction enzyme digestion
   B. End-repair and labelling
   C. In nuclei ligation and reversion of the cross link
   D. DNA purification
   E. Quality controls

3. Hi-C library preparation

   A. DNA Shearing
   B. Biotin Pull-Down and preparation of sequencing libraries
   C. Library amplification and purification
   D. Library quality control and sequencing facts

4. Appendix

   A. Choice of the restriction enzyme
   B. Quality Control
   C. 3C Primers
   D. References

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