1. Purification of FLAG-tagged Protein Complexes from Mammalian Cells
– Protein purification with protein tags
2. Hot Kinase Assay Using Purified F-CDK9 Complex and Autoradiography
– Kinase assay
3. Northern Analysis
– Measuring the size, amount, and abundance of RNA molecules
4. Transient Transfection Assays Using RNAi to Mediated Gene Silencing
– Gene silencing
5.-8. Assays to study chromatin dynamics in vitro: mononucleosome assembly and nucleosome tracking
9. Chromatin Immunoprecipitation followed by qPCR and sequencing
– ChIP-qPCR (known target sequence) & ChIP-seq
10. Genomic data analysis: Methods and Tools
11. Analysis of mRNA expression by Quantitative Real Time PCR (qRT-PCR)
– qRT-PCR
12. Transient Transfection and Reporter Assays to Study Enhancer Activity
– Dual Luciferase Reporter assay
GeneX CSHL 